String Damping:

• Mute: Double-check that the bridge mute (if present) is fully deactivated or removed.
• Palm Muting: Ensure you're not accidentally palm muting the string while playing.
• Fret buzz: Inspect the E string for any buzz against the frets, especially around the 12th fret. Adjust the truss rod slightly if necessary (consult a professional if unsure).

Other Hardware:

• Tuning machine: The E string tuning machine itself could be faulty, causing instability. Try replacing it with a new one.
• Bridge: Check for any loose components in the bridge, especially around the E string saddle. Tighten any loose screws or consult a technician if needed.
• Nut: Inspect the nut slot for the E string. If it's too wide or worn, it can cause oscillation. Consider having a professional luthier cut a new, properly sized slot.

Intonation and Pickup Interference:

• Intonation: Double-check the intonation, especially for the E string. Slight misalignment can create tuning inconsistencies.
• Pickup height: While you lowered the pickups, try raising them slightly (especially the neck and middle pickups) to see if it reduces magnetic pull and oscillation.
• Pickup phasing: If your guitar has multiple pickups, try adjusting their phasing (if possible) to see if it eliminates unwanted interference.

Additional Tips:

• Experiment with string brands and gauges: Different string materials and gauges can have subtle effects on resonance and damping.
• Seek professional help: If you've tried all these suggestions and the problem persists, consult a qualified guitar luthier or technician. They can diagnose the issue more precisely and recommend specific Guitar Parts replacements or adjustments if needed.

Remember, throwing away a guitar should be a last resort. By systematically addressing potential causes and seeking professional help if needed, you can likely get your E string singing true and clear again!

Guitars are like cars sometimes. When things keep going wrong you start to ask why don't I just get a new one? It's frustrating.

Maybe we can help you troubleshoot this. First, you didn't say what kind of guitar you're having this issue with. I'm guessing electric but what make of guitar is it? Next, which e string is giving you the issue - low e or high e?

Also, what kind of tuner are you using?

One thing I always do when I encounter this is mute all the other strings. Lightly touch all the other strings with the palm or fingers until only the string you want is ringing out. This helps the tuner get a clearer reading of what's going on. Why don't you try that and let me know how it goes?